FastQCFastQC Report
Tue 24 Nov 2020
CHM13_Input_1_singles_q20.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCHM13_Input_1_singles_q20.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232554
Sequences flagged as poor quality0
Sequence length100-151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC25340.2055893697152417TruSeq Adapter, Index 4 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19320.15674769624697985No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)