Reconstructing complex regions of genomes using long-read sequencing technology

John Huddleston1,6, Swati Ranade2, Maika Malig1, Francesca Antonacci3, Mark Chaisson1, Lawrence Hon2, Peter H. Sudmant1, Tina A. Graves4, Can Alkan5, Megan Y. Dennis1, Richard K. Wilson4, Stephen W. Turner2, Jonas Korlach2, and Evan E. Eichler1,6

  1. Department of Genome Sciences, University of Washington, Seattle, WA, 98195, USA
  2. Pacific Biosciences of California, Inc., Menlo Park, CA, 94025, USA
  3. Department of Biology, University of Bari, Bari, 70126, Italy
  4. The Genome Institute at Washington University, Washington University School of Medicine, St. Louis, MO, 63110, USA
  5. Department of Computer Engineering, Bilkent University, Ankara, 06800, Turkey
  6. Howard Hughes Medical Institute, University of Washington, Seattle, WA, 98195, USA

BAC sequences

PacBio long reads and HGAP assemblies are available below for all clones. NCBI accession IDs are included for clones with capillary-based assemblies.

Hydatidiform mole (CH17) clones from chromosome 17q21.31

Clone name PacBio reads Illumina reads PacBio GenBank accession Sanger GenBank accession
CH17-124M20 PacBio reads Illumina reads AC254814.1 AC243899.3
CH17-157L1 PacBio reads Illumina reads AC254825.1 AC243627.3
CH17-169A24 PacBio reads Illumina reads AC254822.1 AC243621.2
CH17-170H8 PacBio reads Illumina reads AC254824.1 AC243658.3
CH17-202L17 PacBio reads Illumina reads AC254817.1 AC243659.3
CH17-227A2 PacBio reads Illumina reads AC254818.1 AC243685.2
CH17-33G3 PacBio reads Illumina reads AC254821.1 AC243807.3
CH17-41F14 PacBio reads Illumina reads AC254820.1 AC244207.2

Chimpanzee (CH251) clones from chromosome 17p11.2

Clone name PacBio reads GenBank accession
CH251-545A24 AC183294.3
CH251-12B21 PacBio reads AC254815.1
CH251-21N5 PacBio reads AC254819.1
CH251-354I21 PacBio reads AC254823.1
CH251-433E19 PacBio reads AC254816.1
CH251-59G18 PacBio reads AC254826.1